重組人堿性FGF(成纖維生長因子)
大腸桿菌表達(dá)系統(tǒng)制備，目的基因編碼的Gly132-Ser288表達(dá)純化而來。
FGF Basic質(zhì)量控制：＞95%(還原性SDS-PAGE)。
FGF Basic制劑：凍干品
FGF Basic保存：
凍干蛋白可在-20℃長期保存，室溫也可穩(wěn)定保存3周。
復(fù)溶蛋白溶液可在4～7℃保存2～7天。
復(fù)溶后建議分裝凍存在-20℃，可保存3個(gè)月，應(yīng)避免反復(fù)凍融。
FGF Basic復(fù)溶：
1、試劑開蓋前請先離心。
2、復(fù)溶濃度應(yīng)大于100 μg/ml.
3、凍干蛋白溶于ddH2O中
4、復(fù)溶后根據(jù)使用量分裝保存，避免反復(fù)凍融。
關(guān)于FGF Basic：
FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family.The family constitutes a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4 |
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